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Ph.d. trial lecture and public defence: Qirui Zhang (FBA)


Ph.d.-candidate Qirui Zhang will hold a trial lecture and defend his thesis for the degree Philosophiae Doctor (PhD), at the Faculty of Biosciences and Aquaculture, Nord University.

Start date:

22 February 2019 10:15

End date:

22 February 2019 15:30

Location:

Bodø, Auditorium A13 Elias Blix

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Ph.d.-candidate Qirui Zhang
Title of thesis: 
The effect of embryonic incubation temperature on the immune response of larval and adult zebrafish (Danio rerio)


Title of trial lecture:
Aquaculture related concepts of experimental designs and reports for immunological studies 

Evaluation committee:
- Professor Francesc Piferrer, Institute of Marine Sciences, CSIC, Barcelona, Spain
- Researcher Kaja Skjærven, Institute of Marine Research, Bergen
- Professor Joost Raeymaekers, FBA, Nord University

Supervisors:
Professor Jorge Fernandes, FBA, Nord University
Professor Igor Babiak, FBA, Nord University

About the thesis:
Environmental temperature affects many physiological processes in bony fish, including their immune system. Changes during early development can occur in relation to the varying water temperature. However, little is known about the effect of early developmental temperature on the immune performance of larval and adult fish. 

We used a tropical fish, zebrafish (Danio rerio), as our animal model and incubated embryos at either low (24 °C), high (32 °C) or reference temperature (28 °C). Fish were challenged with lipopolysaccharide from Pseudomonas aeruginosa at first-feeding stage and 100 days post-fertilization, respectively. Significantly different mortality rates and distinct expression of immune-related genes in larvae from different temperature groups were observed. Further transcriptomic (gene expression) analysis revealed a long-term effect of developmental temperature on the immune performance in the spleen of adult zebrafish, with distinct immune transcriptomic patterns between different temperature groups. 

MicroRNAs are a type of small non-coding RNAs that were involved in regulating this long-lasting effect. Taken together, this dissertation demonstrated the effect of early developmental temperature on the immune performance of zebrafish at different life stages. In the context of climate change, it is important to evaluate if fish have the capacity of adapting to the changing temperature, and its potential impact on aquaculture.

The PhD trial lecture and defence are open to the public. The thesis is available on request. Please contact Kristine Vevik, email: kristine.vevik@nord.no

Link for live streaming: https://mediasite.nord.no/Mediasite/Catalog/Full/e2cc911c3d2b4a2284bb0b9728e8be0921



Start date:

22 February 2019 10:15

End date:

22 February 2019 15:30

Location:

Bodø, Auditorium A13 Elias Blix